The relationship between the pressure applied to the enucleated human eye using the Honan Intraocular Pressure Reducer (HIPR) and the peak intraocular pressure as a function of initial intraocular pressure has been examined. The peak intraocular pressure is linearly related to the applied HIPR pressure whether the latter is 30, 50, or 75 mm Hg. The slopes relating peak intraocular pressure to initial intraocular pressure at different HIPR settings are parallel. Use of the HIPR at settings greater than 30 mm Hg and an initial IOP of greater than 30 mm Hg could compromise ocular vascular perfusion.