Automatic DNA sequencer: Computer‐programmed microchemical manipulator for the Maxam–Gilbert sequencing method
作者:
Akiyoshi Wada,
Masahiro Yamamoto,
Eiichi Soeda,
期刊:
Review of Scientific Instruments
(AIP Available online 1983)
卷期:
Volume 54,
issue 11
页码: 1569-1572
ISSN:0034-6748
年代: 1983
DOI:10.1063/1.1137299
出版商: AIP
数据来源: AIP
摘要:
A computer controlled microchemical robot which carries out a series of chemical reaction steps in DNA sequence analysis of Maxam and Gilbert’s scheme is described. Within a 2‐h operation of this apparatus, one of the four types of solutions for G, A+C, C+T, or C analysis for a DNA restriction fragment is prepared and is ready for subsequent electrophoretic analysis. It is proven that the chemical robot operated by a computer command is capable of speedily accomplishing the fragmentation reaction for DNA sequencing with accuracy similar to that of a well‐trained technician.
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