FERRITIN ADSORPTION ON AMOSITE FIBERS: POSSIBLE IMPLICATIONS IN THE FORMATION AND TOXICITY OF ASBESTOS BODIES
作者:
B. Fubini,
F. Barceló,
C. Otero Areán,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1997)
卷期:
Volume 52,
issue 4
页码: 343-352
ISSN:0098-4108
年代: 1997
DOI:10.1080/00984109708984069
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
In order to investigate how endogenous iron can be deposited in vivo on inhaled mineral fibers during early stages of formation of asbestos bodies, in vitro experiments were per formed on the adsorption of ferritin onto amosite asbestos. The mineral dust was found to adsorb the protein from an aqueous solution containing 0.3 mg/ml horse spleen ferritin. In order to simulate physiological conditions the aqueous solution was adjusted with 150 mM saline. Polyacrylamide-SDS gel electrophoresis of the desorbed protein showed sub-units of approximately 13 and 15 kD, aside from the 20-kD subunit present in the native protein. This suggests that as a result of interactions between ferritin molecules and the solid surface of the mineral fibers, the protein iron core may be released or partially exposed. Data indicate these interactions may have implications in the observed mineral fiber toxicities.
点击下载:
PDF (1093KB)
返 回