The preparation of the spacer-containing trimers2, 3-aminopropyl 3-O-[4-O-Me-3-O-(4-N-D,L-lactoyl-3-O-Me-β-D-Quip)-α-L-Rhap]-α-L-Rhap, derivatives of the antigenic determinant of the glycopeptidolipid fromMycobacterium aviumserotype 12, are described. Thus, iodonium ion-mediated glycosylation of the spacer-containing acceptor7with ethyl 1-thio-rhamnopyranoside donor10, followed by selective deprotection of thep-methoxybenzyl group of thus obtained19gave bis-rhamnopyranoside acceptor20. Elongation of20with ethyl 4-azido-1-thio-β-D-quinovopyranoside18and subsequent reduction of the azido function in21led to trimer22. The amino group in22was coupled with both D- and L-lactic acid to give, after removal of the protective groups, trimers2.