Isoxestovanin A (6), xestovanin B (7), xestovanin C (8), dehydroxestovanin A (9), epidehydroxestovanin A (10), dehydroxestovanin C (11), and secodehydroxestovanin A (12), seven new triterpenoid glycosides, have been isolated from the Northeastern Pacific spongeXestospongiavanilla. The structures of the metabolites were determined by a combination of spectroscopic analysis and chemical degradation. The aglycone of6has the new isoxestovanane triterpenoid carbon skeleton. Compounds7,8, and11are the firstX.vanillatriterpenoid glycosides known to contain three monosaccharide subunits.Keywords: triterpenoid, glycoside, sponge, Xestospongia.