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Scanning force microscopy ofE. coliOmpF porin in buffer solution

 

作者: F. A. Schabert,   J. H. Hoh,   S. Karrasch,   A. Hefti,   A. Engel,  

 

期刊: Journal of Vacuum Science&Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena  （AIP Available online 1994）
卷期: Volume 12, issue 3  

页码: 1504-1507

 

ISSN:1071-1023

 

年代: 1994

 

DOI:10.1116/1.587273

 

出版商: American Vacuum Society

 

关键词: ESCHERICHIA COLI;PORINS;BUFFERS;ATOMIC FORCE MICROSCOPY;SURFACE STRUCTURE;SPATIAL RESOLUTION;CONFORMATIONAL CHANGES

 

数据来源: AIP

 

摘要:

The extracellular and periplasmic surface topography of theE.coliouter membrane protein OmpF reconstituted into 2D crystals in the presence of phospholipids were investigated with a scanning force microscope in buffer solution. Topographs exhibiting 2 nm lateral resolution are in good agreement with data from electron microscopy and x‐ray crystallography, and indicate the exciting possibility to monitor structural changes of ‘‘live systems’’ at molecular resolution by scanning force microscopy.  

 

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