Niobium was quantitatively extracted at pri 3-4.5 with 0.08 M Amoerlite LA-2 in xylene from 0.02 M malonic acid. It was stripped from the organic phase with 0.25 M potassium carbonate solution and was determined spectrophotometrically with TAR at 540 nm. Apart from Amberlite LA-2, Primene JM-T quantitatively extracted niobium and xylene were the only satisfactory dilutents. Wiobium was separated from various elements which formed weak complexes with malonic acid by stripping these elements before extraction of niobium. By the process of selective stripping it was separated from elements forming strong complexes. The method was used for the determination of niobium from stainless steel.