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Detection of the red tide-causing planktonAlexandrium affineby a piezoelectric immunosensor using a novel method of immobilizing antibodies

 

作者: Keijiro Nakanishi,   Adachi Masao,   Yoshihiko Sako,   Yuzaburo Ishida,   Hitoshi Muguruma,   Isao Karube,  

 

期刊: Analytical Letters  （Taylor Available online 1996）
卷期: Volume 29, issue 8  

页码: 1247-1258

 

ISSN:0003-2719

 

年代: 1996

 

DOI:10.1080/00032719608001478

 

出版商: Taylor & Francis Group

 

关键词: Alexandrium affine;Monoclonal antibody;IgM;Piezoelectric crystal;Plasma-polymerized Films

 

数据来源: Taylor

 

摘要:

In this paper, the determination ofAlexandrium affinein sea water was performed using piezoelectric immunosensors. In addition, we report a novel method of immobilizing antibodies on the quartz crystal immunosensors using plasma-polymerized ethylenediamine film. The films formed on the quartz crystals are extremely thin, homogeneous and incorporate amino groups. Sensors produced using this method show higher sensitivity than sensors made using conventional immobilization methods for example polyethylenimine or γ-aminopropyltrimethoxysilane.

 

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