A possible role for cysteine proteinase and its inhibitors in motility of malignant melanoma and other tumour cells
作者:
G. Boike,
T. Lah,
B. Sloane,
J. Rozhin,
K. Honn,
R. Guirguis,
M. Stracke,
L. Liotta,
E. Schiffmann,
期刊:
Melanoma Research
(OVID Available online 1992)
卷期:
Volume 1,
issue 5
页码: 333-340
ISSN:0960-8931
年代: 1992
出版商: OVID
关键词: Cystatins;cysteine proteinase;melanoma;motility;tumour
数据来源: OVID
摘要:
The metastasis of malignant tumour cells depends on their rapid replication, and their ability to adhere to the matrix of a biological barrier such as basement membrane, to degrade the matrix, and to migrate through this more permeable barrier. Secreted enzymes, including the cysteine proteinases cathepsins B and L, are known to degrade basement membrane components. Using a barrier-free substratum we studied the possible role of cysteine proteinases in influencing the motilityper seof metastatic cells. We found that stefins, the natural inhibitors of cysteine proteinases, markedly decreased the stimulated motility of both human melanoma cells and W256 carcinosarcoma cells at low concentrations (0.5 μM). A stefin also inhibited melanoma cell adherence, but to a lesser extent than motility. Additionally, synthetic inhibitors (E-64, diazomethyl ketones) of cysteine proteinases were found to depress stimulated motility of W256 cells. These results suggest that cysteine proteinases and their inhibitors may have a direct role in the development of a migratory responseper sein tumour cells.
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