Modification of the Silver Proteinate Impregnation Technique for Protozoa and Cultured Nerve Cells
作者:
VargasConcepción Rugerio,
HurtadoMargarita Mansilla,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 3
页码: 131-135
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110566
出版商: Taylor&Francis
关键词: silver impregnation;silver proteinate;protozoa impregnation;cultured nerve cell impregnation
数据来源: Taylor
摘要:
Silver impregnation with silver-protein compounds is widely used for staining tissue sections and cell cultures. Some authors report that the results obtained with these methods have not always been reproducible because the reagent's composition varies according to the manufacturer. To avoid this problem in the method described in this paper, a silver proteinate, produced in our own laboratory is used. Although our method is based on Bodian's, the modifications we have made allows its use for both free-living cells (protozoa) and cells grown in culture (nerve cells). The significant modifications are 1) different fixation, 2) postfixation with Cajal's for-mol-bromide, 3) changes in the duration of the impregnation steps technique and 4) elimination of metallic copper. The method reported here enables us to use silver proteinate whenever we require it and to control the composition of the silver proteinate. This technique can be used for cells cultured in either plastic or glass.
点击下载:
PDF (1170KB)
返 回