Anopheles quadrimaculatusspecies C1 is a member of the recently describedA. quadrimaculatuscomplex and has been collected from temporary pools in intermittently flooded swamps in north Florida. We examined the hypothesis that species C1 uses persistence in the egg stage as a survival strategy. Species C1 larvae appeared in 40 of 153 samples of dry substrate collected from the floor of four intermittently flooded swamps and flooded with distilled water. Cohorts of first-instar species C1 were collected from temporary pools<24 h after the pools formed and at the same time as first instars of the floodwater mosquitoesAedes infirmatusandPsorophora feroxappeared. In the laboratory, eggs of species C1 were successfully hatched after storage for up to 28 d on moist filter paper. Survival of species C1 eggs allows this species to exploit a niche used by floodwater mosquitoes.