The quantity of rhodopsin in human eyes
作者:
FultonAnne B.,
DodgeJanice,
LynnJeri,
ArmstrongAnnette,
LanierFred,
DawsonWilliam W.,
WilliamsTheodore P.,
期刊:
Current Eye Research
(Taylor Available online 1990)
卷期:
Volume 9,
issue 12
页码: 1211-1216
ISSN:0271-3683
年代: 1990
DOI:10.3109/02713689009003477
出版商: Taylor&Francis
数据来源: Taylor
摘要:
The content of rhodopsin in the eyes of 15 donors (30 eyes) was determined. Both retinal and pigment epithelial fractions were collected from each globe, extracted using 1% CTAB, and the rhodopsin difference spectrum of each fraction was obtained separately. The total amount of rhodopsin, obtained by summing the amounts recovered from the retinal and PE fractions, ranged from 2.00 to 11.94 (median: 6.40) nmoles/eye. Previously reported mean values of about 3.5 to 4.0 nmoles per retina have been obtained using a variety of methods. The present higher values, perhaps largely dependent on procedural details described herein, appear plausible given the known concentrations of rhodopsin in rod outer segments, rod outer segment volumes, and number of rods in the human retina.
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