Intercalation of Pharmorubicin Anticancer Drug to DNA Studied by Cyclic Voltammetry with Analytical Applications
作者:
Chu Xia,
Shen Guoli,
Jiang Jianhui,
Yu Ruqin,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 4
页码: 717-727
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542851
出版商: Taylor & Francis Group
关键词: Cyclic voltammetry;Pharmorubicin anticancer drug;DNA Intercalation
数据来源: Taylor
摘要:
Variations of the cyclic voltammetric behavior of pharmorubicin in an aqueous medium upon addition of DNA were studied and utilized for the determination of DNA. It was observed that the redox waves of pharmorubicin after binding to the large and slowly diffusing DNA almost disappeared. The anodic peak currents of pharmorubicin, when the concentrations were fixed at 2.0 x 10−5and 2.0 x 10−4mol/L, decreased linearly correlated to the concentration of DNA added in the range of 0 - 30.7 and 0 - 260.3 μg/ml, with the detection limits of 1.2 and 20.8 μg/ml, respectively. The proposed method exhibited good recovery and reproducibility for the determination of DNA. Furthermore, the binding constant and binding site size of the pharmorubicin-DNA interaction were also calculated.
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