Development of A Dual Channel Chemiluminescence FIA System for Rapid Measurement of Fish Freshness
作者:
Kenji Hayashi,
Takuji Okugawa,
Yoshiaki Kozuka,
Satoshi Sasaki,
Kazunori Ikebukuro,
Isao Karube,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 14
页码: 2499-2513
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608002787
出版商: Taylor & Francis Group
关键词: fish freshness, flow injection analysis;alkaline phosphatase;thermostable enzyme;inosine 5′-monophosphate;inosine;hypoxanthine
数据来源: Taylor
摘要:
A dual channel chemiluminescence FIA system for the rapid measurement of the fish freshness index KI where [IMP], [HxR] and [Hx] are, respectively, the inosine 5′-monophosphate, inosine and hypoxanthine contents of fish meat was developed. The system consisted of a pair of chemiluminescence FIAs (CL-FIA); one for the measurement of [IMP] + [HxR] + [Hx], and the other for [HxR] + [Hx]. IMP, HxR or Hx were measured using a reactor comprising immobilized alkaline phosphatase, purine nucleoside phosphorylase (PNP) and xanthine oxidase (XOD). The calibration curves for IMP, HxR or Hx were linear from 0.06 μM to 100 μM for a sample volume of 20 μl. The time for a single KI measurement was less than 1 min. This fish freshness FIA system is rapid, convenient and applicable for fish freshness measurements in real fish samples.
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