Rat cardiac neurons express the non‐coding R‐exon (exon 1) of the cholinergic gene locus
作者:
Wolfgang Kummer,
Ludger Fink,
Magdalena Dvorakova,
Rainer Haberberger,
Rainer Bohle,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 10
页码: 2209-2212
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Cardiac ganglia;Cell picking;Choline acetyltransferase;Cholinergic gene locus;Heart;mRNA;Polymerase chain reaction;Rat
数据来源: OVID
摘要:
CHOLINE acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) are both encoded by the cholinergic gene locus which, is the rat, five different species of ChAT mRNA and three different species of VAChT mRNA are produced. So far, discrimination between mRNA subtypes has been possible only in CNS homogenates or in cell cultures. In this study, cardiac neurons were microdissected from frozen sections of rat heart using a u.v. laser and harvested using a micromanipulator. RT-PCR demonstrated the expression of the non-coding R-exon and splicing to R1-type mRNA in the majority of cardiac neurons. The technique presented here is first to allow subtype analysis of cholinergic locus mRNA species in neuronsin situ.
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