Effects of recombinant human tumor necrosis factor (rhtnf) on normal human and mouse hemopoietic progenitor cells
作者:
Kouichi Akahane,
Takayuki Hosoi,
Akio Urabe,
Fumimaro Takaku,
Masanobu Kawakami,
期刊:
The International Journal of Cell Cloning
(WILEY Available online 1987)
卷期:
Volume 5,
issue 1
页码: 16-26
ISSN:0737-1454
年代: 1987
DOI:10.1002/stem.5530050103
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Recombinant tumor necrosis factor;Hemopoietic progenitors;Colony formations
数据来源: WILEY
摘要:
AbstractWe examined the effects of recombinant human tumor necrosis factor (rhTNF) on normal human and murine granulocyte‐macrophage (CFU‐gm) and erythroid (CFU‐e, BFU‐e) progenitor cells. We suppressed in vitro colony formation by human marrow CFU‐gm, CFU‐e and BFU‐e or peripheral blood BFU‐e by adding rhTNF to the culture in a dose‐related manner. A half‐maximal inhibition was observed with 1–10 ng/ml. Leukemic cell line K562 cells were found to be sensitive to rhTNF in the clonogenic colony assay. However, the clonal growth of murine marrow CFU‐e and BFU‐e colonies was less than 50% inhibited and CFU‐gm growth was unaffected even at a concentration of 1,000 ng/ml. We observed slight to moderate inhibition after 24 h pulse exposure of both human and murine‐committed progenitors to rhTNF prior to the culture. Intravenous injection of 1 mg/kg of rhTNF caused a marked decrease in marrow erythroid progenitors and consequently caused anemia in the mice. Our data indicate that rhTNF has a suppressive effect on normal human and murine hemopoietic colony formation in vitro and
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