Evidence for Increased Levels of a Circulating Ouabainlike Factor in Essential Hypertension
作者:
GIUSEPPE SAGNELLA,
JULIA JONES,
ANGELA SHORE,
NIRMALA MARKANDU,
GRAHAM MACGREGOR,
期刊:
Hypertension
(OVID Available online 1986)
卷期:
Volume 8,
issue 5
页码: 433-437
ISSN:0194-911X
年代: 1986
出版商: OVID
关键词: hypertension;Na+-K+pump inhibitor;ouabainlike factor;radioreceptor assay
数据来源: OVID
摘要:
The effect of plasma from normotensive and hypertensive subjects on the binding of [3H]ouabain on human erythrocytes was investigated. The binding of [3H]ouabain on human erythrocytes was saturable and highly specific; linear Scatchard plots indicated the presence of a single type of binding site. Human plasma decreased the binding of [3H Jouabain on its receptor to a greater extent than could be accounted for by the plasma potassium concentration. The level of this circulating ouabainlike factor (or factors) was quantitated using a radioreceptor assay. Plasma from 22 hypertensive subjects (systolic blood pressure > 160 mm Hg or diastolic blood pressure >90 mm Hg) displayed higher levels than that from 24 normotensive subjects; furthermore there was a positive and significant correlation (r = 0.42,n− 46,p< 0.004) between the ouabainlike content and the individual subject's systolic blood pressure. The receptor assay described is relatively simple and should be useful for further work on the nature and clinical importance of the endogenous ouabainlike factor.
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