Comparison of early onco/suppressor gene expressions in peripheral leukocytes and potential target organs of rats exposed to the carcinogen 1-nitropyrene
作者:
I Ember,
I Kiss,
Z Gyöngyi,
Cs Varga,
期刊:
European Journal of Cancer Prevention
(OVID Available online 2000)
卷期:
Volume 9,
issue 6
页码: 439-442
ISSN:0959-8278
年代: 2000
出版商: OVID
关键词: c-myc;gene expression;Ha-ras;leukocytes;Long–Evans rat;1-nitropyrene exposure;oncogene;p53;suppressor gene;surrogate biomarker
数据来源: OVID
摘要:
An in-vivo model has been developed to study early expressions of c-myc, Ha-rasoncogenes andp53suppressor gene as biomarkers of carcinogenic exposure and/or tumorigenesis. In order to validate the in-vivo expression changes as biomarkers, rats were treated with the outdoor air pollutant carcinogen 1-nitropyrene. The gene expression levels were measured after 24 and 48 h in potential target tissues (lung, liver, lymph nodes, kidneys, spleen) and in peripheral blood leukocytes. Another main objective was to prove the applicability of leukocytes as a surrogate tissue, having a similar expression pattern of the selected genes upon carcinogenic exposure. The c-myconcogene was not suitable as an early biomarker because of the lack or low level of its expression. However, in the case of the other oncogene Ha-rasand the suppressor genep53, remarkable and early changes were detected in the expression signals. Similar expression patterns could only be detected in leukocytes and the spleen; therefore we continue this validation study by using other types and routes of exposure.
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