The separation of hop-derived compounds with several high-performance liquid chromatography techniques was studied. A system employing ion-exchange materials was developed which clearly resolvesβ-,α-, and iso-α-acids in less than 2 hr without gradient elution. Adsorption and partition columns can also separate the major groups of hop compounds. Reverse-phase partition chromatography is capable of resolving thecis-andtrans-iso-α-acids.