Staining of rabbit eosinophil and pseudoeosinophil leukocytes
作者:
William M. Shanklin,
Benjamin Sheppard,
George W. Burke, jr.,
期刊:
Cells Tissues Organs
(Karger Available online 1977)
卷期:
Volume 97,
issue 2
页码: 147-150
ISSN:1422-6405
年代: 1977
DOI:10.1159/000144728
出版商: S. Karger AG
关键词: Staining leukocytes;Rabbit
数据来源: Karger
摘要:
Air-dried rabbit blood was stained by HE, PAS and a modification of the Undritz II method. Eosin stained granules red in the eosinophil leukocytes. PAS was negative and the modified Undritz method failed to give consistent results. Cells with eosinophilic granules appeared in the corneal stroma 1 h after removing the corneal epithelium. They were stained red consistently by both eosin and the modified Undritz method. Electron micrographs failed to demonstrate crystalloids in their granules. Because of the staining characteristics and the lack of crystalloids in their granules these cells were classified as pseudoeosinophil leukocytes. The electron micrographs showed some glycogen 12 h after denuding the cornea, however, glycogen was not well stained by PAS until 18 h after denuding.
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