A Pre-embedding Reaction Method for Localizing NADH Reductase and Succinic Dehydrogenase in Skeletal Muscle
作者:
HawcroftD.,
BallM.,
BurrowsJ.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 3
页码: 136-139
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110023
出版商: Taylor&Francis
关键词: oxidoreductase localization;pre-embedding reaction;paraffin wax sections
数据来源: Taylor
摘要:
Paraffin wax embedding methods suitable for demonstrating the distribution of enzyme activity in tissues sections are uncommon; most procedures rely on the use of frozen section techniques. This paper describes a system for demonstrating certain enzymes which involves incubation of the tissue with appropriate substrates before a Paramat wax embedding procedure. While it has distinct merits of its own, the procedure is eminently suitable for use where a cryostat is not available; it can also be readily applied to other enzymes and tissues.
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