Attachment of apseudomonassp. to Fe(III)‐(hydr)oxide surfaces
作者:
JenniferH. Forsythe,
PatriciaA. Maurice,
LarryE. Hersman,
期刊:
Geomicrobiology Journal
(Taylor Available online 1998)
卷期:
Volume 15,
issue 4
页码: 293-308
ISSN:0149-0451
年代: 1998
DOI:10.1080/01490459809378084
出版商: Taylor & Francis Group
关键词: AFM;bacteria;biofilm;hematite;iron;goethite;Pseudomonas;SPM
数据来源: Taylor
摘要:
As a first step towards understanding microbial dissolution processes, our research focuses on characterizing attachment features that form between aPseudomonassp. bacteria and the Fe(III)‐(hydr)oxide minerals hematite and goethite. Microbial growth curves in Fe‐limited growth media indicated that the bacteria were able to obtain Fe from the Fe(III)‐(hydr)oxidesfor use in metabolic processes. A combination of scanning electron microscopy, epifluorescence, and Tapping Mode™ atomic‐force microscopy showed that the bacteria colonized some fraction of mineralogical aggregates. These aggregates were covered by bacteria and were linked together by relatively open biofilms consisting of networks of fiber‐like attachment features intertwined through thin films of amorphous‐looking organic material. The biofilm material encompassed numerous individual bacteria, as well as minéralogie particles. We hypothesize that the bacteria first attached to mineral aggregates, perhaps via their flagella, forming colonies. Following initial attachment, the bacteria exuded additional attachment features in the form of fine, branching fibrils intertwined through thin films. The detailed structures of these attachment features were highlighted by Phase Imaging atomic‐force microscopy, which served as a real‐time contrast enhancement technique and showed some poorly defined sensitivity to different surface materials, most probably related to differences in stiffness or viscoelasticity. Although the mechanism of the microbially enhanced dissolution remains unknown, we hypothesize that the bacteria may have produced micro environments conducive to dissolution through the use of observed extracellular materials.
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