Analysis of antiepileptic drugs by capillary gas chromatography with on‐column injection
作者:
Juraj Volmut,
Miloslav Melník,
Eva Matisová,
期刊:
Journal of High Resolution Chromatography
(WILEY Available online 1993)
卷期:
Volume 16,
issue 1
页码: 27-30
ISSN:0935-6304
年代: 1993
DOI:10.1002/jhrc.1240160105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Capillary GC;Solid phase extraction;Cold on‐column injection;Antiepileptic drugs
数据来源: WILEY
摘要:
AbstractA simple and quick gas chromatographic method has been developed for the determination of up to six commonly used antiepileptic drugs in human serum. The antiepileptics are isolated from serum by solid phase extraction on to a reversed phase sorbent and recovered with ethyl acetate as eluent. The ethyl acetate eluate is suitable for direct on‐column injection on to a phenyl methyl siloxane capillary column; hydrogen is used as carrier gas and the compounds are separated with a two‐ramp temperature program. Detection is by FID.The reproducibility of the method varies between 2 and 6% RSD, depending on the drug and the level analyzed; limits of detection were found to be 14–51 pg and minimum assayed concentrations in serum were between 14 and 51
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