Interaction between Wheat-Germ Agglutinin and acid phosphatase present in dry de-embryonated wheat grains
作者:
Giovanna Carratu,
Michelangelo Melchionna,
Matteo Giannattasio,
期刊:
Giornale botanico italiano
(Taylor Available online 1983)
卷期:
Volume 117,
issue 5-6
页码: 219-226
ISSN:0017-0070
年代: 1983
DOI:10.1080/11263508309427971
出版商: Taylor & Francis Group
关键词: Acid phosphatase;WGA;wheat grains
数据来源: Taylor
摘要:
Different hydrolases were found to be present in the crude extract of dry de-embryonated wheat grains, including acid phosphatase. When the crude extract was chromatographed on a Wheat-Germ Agglutinin-Sepharose affinity column, an aliquot of acid phosphatase bound to the column and could be eluted specifically by N-acetyl-D-glucosamine solution. Some properties of the WGA-binding acid phosphatase (pH optimum, heat stability, Triton X-100 sensitivity, inhibition by some ions, molecular weight and Km) were studied. WGA appeared to have a stabilizing effect on the enzyme, while it was ineffective on the Km.
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