Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails
作者:
Toshinori Sato,
Hiroyuki Akino,
Yoko Shoji,
Jingoro Shimada,
Yoshio Okahata,
期刊:
Journal of Biomaterials Science, Polymer Edition
(Taylor Available online 1998)
卷期:
Volume 9,
issue 1
页码: 31-42
ISSN:0920-5063
年代: 1998
DOI:10.1163/156856297X00245
出版商: Taylor & Francis Group
关键词: DNA;lipoglutamide;DNA complex;poly(ethylene glycol);gene transfer;subcellular distribution;confocal laser scanning microscope
数据来源: Taylor
摘要:
—Lipoglutamides with tetraethylene glycol tails were synthesized. Physicochemical features of the DNA/lipoglutamide complexes were investigated by light scattering method, phase transition, and CD-spectrum. Aggregation of the DNA/lipoglutamide complex was significantly depressed compared with DNA complexes without ethylene glycol tails, and the solution showed no turbidity. The DNA/lipoglutamide complex showed a high resistance to nuclease, and an efficient internalization into tumor cells, compared with those of DNA alone. Furthermore, the DNA complex was found by confocal laser scanning fluorescence microscopy to distribute in the cytoplasmic region.
点击下载:
PDF (1953KB)
返 回