Partial cloning and distribution of estrogen receptor beta in the avian brain
作者:
Bernard Lakaye,
AgnèAs Foidart,
Thierry Grisar,
Jacques Balthazart,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 12
页码: 2743-2748
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Coturnix japonica;Estrogen receptor beta;ER-β;Japanese quail;Hypothalamus;In situhybridization;Preoptic area;Sequencing
数据来源: OVID
摘要:
A partial estrogen receptor beta (ER-β) cDNA was isolated from testicular quail RNA by RT-PCR with degenerate primers specific to the rat ER-β sequence. A high expression of ER-β was demonstrated by RT-PCR in the telencephalon, diencephalon, pituitary, testis and kidneys of male quail but little or no expression was detected in the cerebellum, pectoral muscle and adrenal gland.In situhybridization with a35S-labelled oligoprobe in sections through the preoptic area-rostral hypothalamus identified high expression in the medial preoptic nucleus, bed nucleus striae terminalis and nucleus taeniae. These data demonstrate the presence of an ER-β in brain areas implicated in the control of reproduction in a non-mammalian species.
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