Sera from Jra Patients Contain Antibodies Against a Defined Epitope in Chromosomal Protein HmG-17
作者:
NeuerGunter,
BautzFriedlinde A.,
BustinMichael,
MichelsHartmut,
TruckenbrodtHans,
期刊:
Autoimmunity
(Taylor Available online 1994)
卷期:
Volume 17,
issue 1
页码: 23-30
ISSN:0891-6934
年代: 1994
DOI:10.3109/08916939409014655
出版商: Taylor&Francis
关键词: juvenile rheumatoid arthritis (JRA);high mobility group protein (HMG);epitope mapping;ELISA
数据来源: Taylor
摘要:
Autoantibodies against the nonhistone nucleosomal protein HMG-17 have been detected in a high percentage of ANA-positive patients with pauciarticular-onset JRA4. Here we report on the epitope mapping of the HMG-17 autoantigen with a set of overlapping and nested synthetic peptides spanning the entire amino acid sequence of the human HMG-17 protein. Competition ELISA experiments defined a proline and lysine rich octapeptide PKPEPKPK as the major epitope recognized by more than 70 % of the HMG-17 positive JRA sera. Point mutations introduced in the autoimmune peptide determined the amino acid residues important for autoantibody recognition. Computer based sequence comparison shows close homology between the HMG-17 autoimmune epitope and certain infectious organisms, supporting the possibility that molecular mimicry is an important factor in the etiology of JRA.
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