CARDIAC ALLOGRAFT SURVIVAL ACROSS MAJOR HISTOCOMPATIBILITY COMPLEX BARRIERS IN THE RHESUS MONKEY FOLLOWING T LYMPHOCYTE‐DEPLETED AUTOLOGOUS MARROW TRANSPLANTATIONIV. IMMUNE RECONSTITUTION
作者:
ROBERT MOSES,
SUSAN SHARROW,
DAVID STEPHANY,
KATHRYN ORR,
RONALD GRESS,
期刊:
Transplantation
(OVID Available online 1989)
卷期:
Volume 48,
issue 5
页码: 774-781
ISSN:0041-1337
年代: 1989
出版商: OVID
数据来源: OVID
摘要:
Studies of postmyeloablative immune reconstitution have been reported for allogeneic bone marrow transplantation and also for non-T cell-depleted autologous/syngeneic BMT. However, there is a paucity of information regarding immune recovery following T cell-depleted autologous/syngeneic BMT. We have developed a primate transplantation tolerance model in which rhesus monkeys were conditioned with total-body irradiation and extensively T cell-depleted autologous BMT and given a major histocompatibility complex—mismatched heterotopic cardiac allograft. This model provided an opportunity to study peripheral immune recovery following T cell-depleted autologous BMT. Limiting dilution analysis was used to quantify marrow T cells following depletion (2.8% to 25.6% marrow T cells predepletion, 0.00014% to 0.036% residual marrow T cells postdepletion). We found that (1) hematopoietic engraftment was prompt despite extensive marrow T cell depletion, (2) reconstitution of CD4+helper T cells and CD8+cytotoxic T cells were substantially delayed (6–12 months) compared with the recovery of CD8+suppressor T cells, CD16+NK cells, and CD20+B cells, (3) distinction between CD8+cytotoxic T cells and CD8+suppressor T cells by the CD28 marker was critical in revealing the markedly discrepant recoveries of those subsets, and (4) immune reconstitution resembled that observed in recipients of T cell-depleted allogeneic and non-T cell-depleted autologous/syngeneic BMT, suggesting that the pattern of immune recovery following BMT is not substantially influenced by either allogeneic effects or the number of transferred T cells over a range of values.
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