Different cultivars of grape plants were used in these experiments. They included 3‐month‐old plants obtained by a method of clonal micro‐reproduction, seedlings grown in pots from 2‐budded cuttings, and perennial fruit‐bearing bushes used in vineyards. Iron (Fe) concentration in grape plants varied depending on tissue, varieties, stage of vegetative growth, condition of the plants, growing conditions, etc. Iron deficiency resulted in chlorosis and Fe content decreased in leaves from plants growing in an artificial nutrient medium. As chlorosis progressed in severity, it resulted in a decrease in Fe content in the leaves and annual shoots. This might be connected with premature senescence of plants and Fe efflux into perennial tissue in spite of its relatively low mobility in plants. Foliar supplement with Fe‐containing compounds to chlorotic plants increased the Fe content of leaves and improved the general health of the plants.