Adaptation of the EMIT® Gentamicin and Tobramycin Procedure to the IL Microcentrifugal Analyzer
作者:
Mariet Iosefsohn,
Roger Boeckx,
Jocelyn Hicks,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1981)
卷期:
Volume 3,
issue 4
页码: 365-370
ISSN:0163-4356
年代: 1981
出版商: OVID
关键词: : Gentamicin and Tobramycin procedure;IL microcentrifugal analyzer;EMIT®
数据来源: OVID
摘要:
We have adapted the Syva Emit® gentamicin and tobramycin procedure to the IL microcentrifugal analyzer, MCA III®. With this instrument the recommended instructions for reagent preparation and reaction sequence did not produce a useable standard curve. We modified the dilutions and volumes for the working reagents and reversed the sequence of addition of the working reagents. We assessed the linearity and precision of the analysis and compared the Syva gentamicin and tobramycin Emit procedure on the IL MCA III with the semiautomated procedure on a Gilford Stasar III spectrophotometer. With the IL MCA III® only 3 to 5μ of serum is used, 17 analyses can be obtained in 8 min, and the reagent costs are reduced considerably.
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