A quantitative analysis of the accuracy ofIn VivopH measurements with31P NMR spectroscopy: Assessment of pH measurement methodology
作者:
A. Madden,
M. O. Leach,
J. C. Sharp,
D. J. Collins,
D. Easton,
期刊:
NMR in Biomedicine
(WILEY Available online 1991)
卷期:
Volume 4,
issue 1
页码: 1-11
ISSN:0952-3480
年代: 1991
DOI:10.1002/nbm.1940040102
出版商: John Wiley&Sons, Ltd.
数据来源: WILEY
摘要:
Abstract31P NMR spectroscopy provides a means of monitoring intracellular pH (pHi). In order to determine the significance of observed pH changes, an assessment of the measurement uncertainty is required. This paper considers the possible sources of error involved, firstly in chemical shift assignment and, secondly, in relating chemical shift to pH. A statistical analysis of these errors is presented and an expression for the accuracy of pH measurements obtained usingin vivo31P NMR spectroscopy is derived. Computer simulations and clinical measurements are considered to determine the likely error in clinical measurements. A comparison of pH measurement methodology using a centre‐of‐gravity calculation, Gaussian and Lorentzian fitting is also presented and discus
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