Stereoselective Determination of Fluoxetine and Norfluoxetine Enantiomers in Plasma Samples by High-Performance Liquid Chromatography
作者:
Simona Pichini,
Roberta Pacifici,
Ilaria Altieri,
Manuela Pellegrini,
Piergiorgio Zuccaro,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 12
页码: 1927-1935
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608014016
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The simultaneous liquid chromatographic determination of the enantiomers of both fluoxetine and its metabolite norfluoxetine in plasma samples of treated patients is described. The compounds are subjected to solid phase extraction before chromatography. The separation of the analytes is achieved using two chiralcel ODR columns on-line coupled and a mobile phase consisting of acetonitrile-NaClO40.3M (66/34 v/v, pH 2.5) at a flow rate of 0.6 mL/min. The compounds were detected by ultraviolet absorbance at 220 nm. The limit of quantification for each compound was 10ng/mL.
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