U46619‐mediated vasoconstriction of the fetal placental vasculaturein vitroin normal and hypertensive pregnancies
作者:
Mark Read,
Ian Leitch,
Warwick Giles,
Andrew Bisits,
Alan Boura,
William Walters,
期刊:
Journal of Hypertension
(OVID Available online 1999)
卷期:
Volume 17,
issue 3
页码: 389-396
ISSN:0263-6352
年代: 1999
出版商: OVID
关键词: thromboxane;placenta;pre-eclampsia;U46619;hypertension;prostacyclin;pregnancy
数据来源: OVID
摘要:
ObjectivesTo measure in-vitro responses to the thromboxane A2(TxA2) mimetic U46619 in the fetal placental vasculature of human placentae from normotensive women and those with pre-eclampsia. Furthermore, to compare fetal vascular responses to endothelin-1, 5-hydroxytryptamine, potassium chloride (KCl) and prostacyclin (PGI2) in placentae from normal or pre-eclamptic pregnancies.MethodsSingle placental lobules of intact placentae were bilaterally perfusedin situ(fetal and maternal) with constant flows of Krebs' solution. Changes in fetal arterial perfusion pressure during intra-arterial infusion of vasoactive agents were recorded. Fetal placental vasoconstrictor concentration response curves were obtained to U46619 (0.01–300 nmol/l), endothelin-1 (0.4–160 nmol/l), KCl (3–300 mmol/l) and 5-hydroxytryptamine (0.03–30 μmol/l). In addition, vasodilator concentration response curves were obtained for PGI2(1.2–350 nmol/l) in the fetal placental circulation during submaximal increases in perfusion pressure with prostaglandin F2α(PGF2α; 0.7–2.0 μmol/l).ResultsThe maximum increase in perfusion pressure caused by U46619 in placentae from normotensive women was 194 ± 25 mmHg. The maximum response to U46619 was significantly reduced in the placentae from women with pre-eclampsia (104 ± 21 mmHg). In contrast, there were no differences in constrictor responses to endothelin-1, 5-hydroxytryptamine and KCl, or in dilator responses to PGI2in placentae obtained from either normotensive women or those with pre-eclampsia.ConclusionTxA2receptor-mediated vasoconstriction is reduced in the fetal vasculature of placentae from women with pre-eclampsia, possibly to compensate for the increased levels of TxA2seen in these conditions.
点击下载:
PDF
(224KB)
返 回