Testosterone Determination Using Rapid Heterogeneous Competitive-Binding for Enzyme-Linked Immunosorbent Assay in Flow Injection.
作者:
LucyT. Di Benedetto,
Telis Dimitrakopoulos,
RayM. Davy,
PeterJ. Iles,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 12
页码: 2125-2139
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608002236
出版商: Taylor & Francis Group
关键词: Testosterone;Enzyme-linked immunosorbent assay;ELISA protocol;Incubation time;Flow injection
数据来源: Taylor
摘要:
The application of a rapid competitive enzyme-linked immunosorbent assay (ELISA) for the measurement of testosterone in flow injection (FI) is presented. The ELISA protocol for the determination of testosterone was performed in 60 min compared to the standard protocol time of 150 min. This was achieved by reducing the antibody incubation time. The ELISA was used successfully to determine testosterone levels in clinical samples of both male and female subjects, and were in good agreement compared to a pathology laboratory which employed an immunoradiometric assay (IRMA).
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