Selective MR imaging of labeled human peripheral blood mononuclear cells by liposome mediated incorporation of dextran‐magnetite particles
作者:
Jeff W. M. Bulte,
Loralie D. Ma,
Richard L. Magin,
Richard L. Kamman,
Caesar E. Hulstaert,
K. Gwan Go,
T. Hauw The,
Lou De Leij,
期刊:
Magnetic Resonance in Medicine
(WILEY Available online 1993)
卷期:
Volume 29,
issue 1
页码: 32-37
ISSN:0740-3194
年代: 1993
DOI:10.1002/mrm.1910290108
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Labelingex vivo;lymphocytes, monocytes;magnetic liposomes;MR contrast agent
数据来源: WILEY
摘要:
AbstractHuman peripheral blood mononuclear cells (PBMCs) were incubated with large unilamellar vesicles (LUV) containing encapsulated dextran‐magnetite particles (DMP). This resulted in an efficient incorporation of DMP. Electron microscopy revealed the presence of DMP in cells mainly in phagosomes and secondary lysosomes. DMP‐labeled PBMCs showed a strong increase of the transverse relaxation rate (up to 16.6 s−1for 5 x 107cells/ml) and, accordingly, a great loss of signal intensity in MR imaging. The fraction of DMP containing PBMCs could be enriched by magnetic cell separation. The major population of the DMP containing cells proved to be monocytes. When PBMCs depleted of monocytes were used for labeling, DMP uptake was observed also in the peripheral blood lymphocytes. The labeling of PBMCs presented here may be used in future studies of selective MR imaging ofin vivocell migration in a variety of immunologically compromised tissue states, e.g., tumors, transplantations, and absc
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