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Hb F-Tokyo orα2GΓ234(B16) Val→Ile, A SilentΓChain Variant Detected by Reverse Phase high Performance Liquid Chromatography

 

作者: ChenS. S.,   WilsonJ. B.,   WebberB. B.,   HuismanT. H. J.,   MiwaS.,   AmenomoriY.,  

 

期刊: Hemoglobin  （Taylor Available online 1985）
卷期: Volume 9, issue 1  

页码: 25-32

 

ISSN:0363-0269

 

年代: 1985

 

DOI:10.3109/03630268508996979

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

Hb F-Tokyo with a Valine→Isoleucine replacement at positionγ34(B16) is a Gγchain variant which was discovered by reverse phase chromatography as this method permitted the nearly complete separation of the three types ofγchain. The chemical characterization was greatly facilitated by the use of a larger, preparative, HPLC column which allowed the isolation of sufficient quantities of the differentγchains.

 

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