Coordination compounds of Zn(II), Cu(II) and Ni(II) with N-[5-nitro-furfurylidene]-N2-[β-isatin]azine were prepared. Using elemental analysis, atomic absorption (AA), molar conductances, Fourier transform infrared (FTIR), electronic (UV/VIS) spectroscopics methods, their structure were established to be [ZnLCl2].H2O, [CuLCl2].H2O and [NiL2Cl2].2H2O. Spectral studies indicate complexation through nitrogen aiom and oxygen atom of isatin part of ligand. Coordination compounds were tested for antibacterial activity againstStaphylococcus aureus. Enterococcus D. Proteus vulgaris and Escherichia coli.