Fluorimetric Determination of Thiamine and Cocarboxylase by a Differential Rate Method
作者:
V. González,
S. Rubio,
A. Gómez-Hens,
D. Pérez-Bendito,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 6
页码: 993-1008
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808071926
出版商: Taylor & Francis Group
关键词: Fluorimetry;differential kinetic method;thiamine;cocarboxylase;pharmaceuticals
数据来源: Taylor
摘要:
A differential kinetic fluorimetric method is proposed for the simultaneous determination of thiamine (vitamin B) and its pyrophosphate ester (cocarboxylase). It is based on the oxidation of these analytes by mercury(II) in a basic medium to yield fluorescent thiochrome. The calibration graph of each analyte is linear in the range 4 × 10−8−3 × 10−5M. The logarithmic extrapolation method is used to resolve thiamine/cocarboxylase mixtures in ratios between 4:1 and 1:15. Results of precision and selectivity studies are reported. Thiamine and cocarboxylase are satisfactorily determined in synthetic multivitamin preparations by the proposed method. A method for the individual determination of cocarboxylase is also described and applied to the analysis of this analyte in commercial pharmaceutical preparations.
点击下载:
PDF (453KB)
返 回