32‐msec scan of the NAD(P)H fluorescence spectrum in single living cells
作者:
Elli Kohen,
Cahide Kohen,
Bo Thorell,
Jean‐Marie Salmon,
期刊:
Review of Scientific Instruments
(AIP Available online 1973)
卷期:
Volume 44,
issue 12
页码: 1784-1785
ISSN:0034-6748
年代: 1973
DOI:10.1063/1.1686056
出版商: AIP
数据来源: AIP
摘要:
A multichannel microspectrofluorometer based on an electron bombardment silicon camera tube, a multiscaling computer, and a miniature Amici prism for spectrometer, allows the scanning of the NAD(P)H fluorescence emission spectrum from a 30 &mgr; region in a single living EL2 ascites cancer cell, within 32 msec. Observations of changes in the relative maximum emission intensities of two fluorochromes within a same cell are quite possible (for emission maxima ∼ 10–20 nm apart). Thus, in the fluorescence emission spectrum of cells incubated with benzpyrene or dibenzanthracene, the 423 nm (polycyclic hydrocarbon)/443 nm [NAD(P)H] ratio is 1.25 or over, while it decreases to 1.0 or less upon microelectrophoretic addition of glucose‐6‐phosphate [NAD(P) reduction].
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