Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Methods used and chemicals evaluated
作者:
Ann D. Mitchell,
Brian C. Myhr,
Colette J. Rudd,
William J. Caspary,
Virginia C. Dunkel,
期刊:
Environmental Mutagenesis
(WILEY Available online 1988)
卷期:
Volume 12,
issue S13
页码: 1-18
ISSN:0192-2521
年代: 1988
DOI:10.1002/em.2860120502
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: L5178Y mouse lymphoma cells;mammalian cell mutagenesis assay;thymidine kinase locus;trifluorothymidine resistance;chemical mutagen screening
数据来源: WILEY
摘要:
AbstractA general protocol, modified from the one described by Clive and Spector (Mutat Res 31:17–29, 1975), was followed by two laboratories, Litton Bionetics, Inc., and SRI International, to evaluate 63 coded chemicals from 16 chemical classes for mutagenic activity at the thymidine kinase locus in L5178Y TK±3.7.2C mouse lymphoma cells. The general protocol is discussed. Some procedural variations introduced by both laboratories are described and discussed in terms of their potential effect on the comparative results of the assay. Also included are the chemical structures, molecular weights, and functional classifications of the 63 chemicals. The assay appeared to tolerate the specific procedural variations in each laboratory without changing its reliabili
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