Effect ofN‐methyl‐N′‐Nitro‐N‐nitrosoguanidine on amino acid incorporation into mucosal protein of rat stomach
作者:
R. S. Bagewadikar,
R. K. Bhattacharya,
期刊:
Journal of Biochemical Toxicology
(WILEY Available online 1992)
卷期:
Volume 7,
issue 1
页码: 19-24
ISSN:0887-2082
年代: 1992
DOI:10.1002/jbt.2570070105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: N‐methyl‐N′‐Nitro‐N‐Nitrosoguanidine;Stomach Mucosa;Amino Acid Incorporation;Polyribosome;Toxicity
数据来源: WILEY
摘要:
AbstractAdministration of the carcinogenic N‐nitroso compound,N‐methyl‐N′‐nitro‐N‐nitrosoguanidine in drinking water (0.5 mg/mL) to male Wistar rats for 1 week caused impairment of in vivo and in vitro incorporation of [14C]leucine into stomach mucosal protein. This impairment gradually returned to normal after 4 weeks. Uptake of [14C]leucine into mucosal protein was significantly inhibited after in vitro treatment of stomach mucosa with the carcinogen. Addition of theN‐nitroso compound in a cell‐free system using postmitochondrial supernatant prepared from stomach mucosa also showed inhibition of amino acid incorporation. Using a more defined system consisting of purified polyribosome from stomach mucosa and pH 5 enzyme fraction derived from liver it was further demonstrated that the carcinogen purturbed protein synthesizing ability of polyribosome, under both in vivo and in vitro treatment conditions. In these respects this carcinogen has similar action on the target tissue of stomach as in the liver, although the in vivo effect may be related more to toxicity tha
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