Subtype‐specific problems with quantification of plasma HIV‐1 RNA
作者:
Annette Alaeus,
Knut Lidman,
Anders Sönnerborg,
Jan Albert,
期刊:
AIDS
(OVID Available online 1997)
卷期:
Volume 11,
issue 7
页码: 859-865
ISSN:0269-9370
年代: 1997
出版商: OVID
关键词: Genetic subtype;quantification;viral load;plasma RNA level;polymerase chain reaction;HIV monitor;nucleic acid sequence-based amplification
数据来源: OVID
摘要:
Objective:To determine whether two commercial assays for quantification of plasma HIV-1 RNA levels detect different genetic subtypes of HIV-1 with equal efficiency.Design:Blind testing of stored plasma samples from 95 individuals infected with different genetic subtypes of HIV-1 (27 subtype A, 24 B, 18 C, 18 D, two E, two G, two H, and two J). The HIV-1 subtype had previously been determined by direct sequencing of the V3 domain of theenvgene.Methods:One plasma sample from each individual was tested once by the Roche HIV monitor assay and once by the Organon nucleic acid sequence-based amplification (NASBA) HIV-1 RNA quantitative assay, according to the manufacturers' recommendations. Information about CD4+ lymphocyte counts and antiretroviral treatment was available.Results:The results from the two assays were strongly correlated with each other for subtypes B, C and D, but not for subtype A because many samples had RNA levels close to or below the lower detection limit of the assays. Thus, 15 out of 27 (56%) subtype A samples were negative by the HIV monitor assay and 12 (44%) were negative by the NASBA assay. These frequently occurring negative results among subtype-A-infected individuals were not due to better immunological status, more aggressive antiretroviral treatment, or differences in sample storage conditions.Conclusions:The HIV monitor assay and, possibly to slightly lesser degree, the NASBA assay appear unable to accurately quantify HIV-1 RNA levels in plasma samples from many subtype-A-infected individuals. These problems are likely to be due to primer mismatches and they limit the possibility of using these assays for routine monitoring of HIV-1-infected individuals in many parts of the world.
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