Low-Molecular-Weight Protein Competition for Binding Sites on Renal Brush Border Membranes
作者:
Albert W. Dreisbach,
Vecihi Batuman,
期刊:
Kidney and Blood Pressure Research
(Karger Available online 1994)
卷期:
Volume 17,
issue 6
页码: 287-293
ISSN:1420-4096
年代: 1994
DOI:10.1159/000173844
出版商: S. Karger AG
关键词: Brush border membrane;Immunoglobulin light chains;Low-molecular-weight proteins;binding;β2-Microglobulin
数据来源: Karger
摘要:
Immunoglobulin light chains, β2-microglobulin, insulin, and lysozyme are low-molecular-weight proteins (LMWP) shown to bind to renal brush border membranes. Competition among these proteins and the role of electrical charge in binding to brush border membranes have not been resolved. To investigate these factors, we performed displacement experiments with [125I]-labeled β2-microglobulin (pI - 5.6) using six species of LMWP over a pi range of 4.4-11.0. The inhibition constants, Ki, of these six competing ligands, χ- and λ-light chains, lysozyme, insulin, cytochrome c, and myoglobin, determined from the log displacement curves, ranged from 4×10-5 to 8×10-4M. These experiments show marked cross-competition among LMWP for binding to brush border membranes. There was no correlation between Ki and pI indicating that the molecular structure is a more important determinant of LMWP binding to brush border membranes than net electrical c
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