Results are described of a split‐udder trial on the effect of discontinuation of postmilking teat disinfection on the incidence of clinical mastitis in seven dairy herds with a low bulk milk somatic cell count and a high incidence of clinical mastitis. Overall incidence of clinical mastitis was non‐significantly lower (18%), whereas the incidence of the most prevalent pathogen associated with clinical mastitis,Escherichia coli,was significantly lower in quarters for which postmilking teat disinfection was discontinued. We concluded that discontinuation of postmilking teat disinfection may decrease the incidence of clinicalEscherichia colimastitis in herds for which standard mastitis prevention measures are executed adequately, bulk milk somatic cell count is low, and incidence of clinical mastitis is high. However, because an increase in intramammary infections with contagious pathogens may occur, care is recommended when advising discontinuation of postmilking teat disinfection.