FLUORESCENCE CONCENTRATION IMMUNOASSAY FOR RAPID DETECTION OFCAMPYLOBACTERSPP. IN CHICKEN RINSE WATER
作者:
HSIU‐CHUAN SONIA TSAI,
MICHAEL F. SLAVIK,
期刊:
Journal of Rapid Methods&Automation in Microbiology
(WILEY Available online 1994)
卷期:
Volume 3,
issue 1
页码: 69-76
ISSN:1060-3999
年代: 1994
DOI:10.1111/j.1745-4581.1994.tb00309.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
ABSTRACTA total of 215 freshly processed post‐chill whole chicken carcasses were assessed for Campylobacter spp. contamination by a fluorescence concentration immunoassay (FCIA) procedure. Whole chicken carcasses were sampled with low volume water rinses from which 5 ml portions were enriched with brucella enrichment broth with or without oxyrase supplement in a test tube enrichment system. After a 24h stationary incubation at 42C, each sample was assayed using a FCIA procedure for the presence or absence of campylobacters. The FCIA procedure indicated Campylobacter spp. contamination in 84% of carcasses using oxyrase supplemented enrichment, while only 47% of the chicken carcasses were positive from nonsupplemented enrichment. The corresponding incidence rates detected by culture method were 92% and 87% for oxyrase supplemented and unsupplemented samples, respectively. The FCIA procedure can be completed in less than 1 h with 48 samples including a positive and a negative control assayed on one plate. In summary, the test tube oxyrase‐supplemented stationary enrichment system followed by the use of the FCIA procedure was found to be an effective, rapid method for the detection of Campylobacter spp. in chicken rinse wa
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