Chromatin organization in isolated nuclei: Flow cytometric characterization employing forward and perpendicular light scatter
作者:
Stefano Papa,
N. M. Maraldi,
A. Matteucci,
P. Santi,
M. Vitale,
A. Galanzi,
F. A. Manzoli,
期刊:
Cell Biochemistry and Function
(WILEY Available online 1988)
卷期:
Volume 6,
issue 1
页码: 31-38
ISSN:0263-6484
年代: 1988
DOI:10.1002/cbf.290060106
出版商: John Wiley&Sons, Ltd.
关键词: Perpendicular light scatter;forward light scatter;chromatin;flow cytometry;electron microscopy
数据来源: WILEY
摘要:
AbstractFlow cytometric perpendicular and forward light scatters have been employed to evaluate whether the changes in chromatin organization due to ionic strength, Mg++concentration and pH, visible in electron microscopy, can be monitored by flow cytometry.The average intensity of the perpendicular light scatter signal increased as nuclear chromatin became decondensed by lowering the ionic strength or releasing H1 histone at low pH values.These results indicate that flow cytometry signals and in particular the perpendicular light scatter allow the detection of the conformational transitions in chromatin and may therefore be useful for studying cell cycle associated morphological changes in isolated nuclei.
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