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Alpha-Fetoprotein as a TNF Resistance Factor for the Human Hepatocarcinoma Cell Line HepG2

 

作者: Lydia N. Semenkova,   Elena I. Dudich,   Igor V. Dudich,   Ludmila N. Shingarova,   Vyacheslav G. Korobko,  

 

期刊: Tumor Biology  (Karger Available online 1997)
卷期: Volume 18, issue 1  

页码: 30-40

 

ISSN:1010-4283

 

年代: 1997

 

DOI:10.1159/000218013

 

出版商: S. Karger AG

 

关键词: α-Fetoprotein;Tumor necrosis factor;Cytotoxicity;HepG2 cells

 

数据来源: Karger

 

摘要:

Human hepatocarcinoma HepG2 cells are known to be insensitive to tumor necrosis factor (TNF) cytotoxicity. In this report, preliminary washing of HepG2 cells with serum-free medium to remove endogenous and exogenous α-fetoprotein (AFP) from the cultivation medium transfers cells from the TNF-resistant to the TNF-sensitive state without addition of any transcriptional inhibitors. HepG2 cells sensitized to by washing again became TNF-resistant after their treatment with exogenous AFP. Protective AFP activity against TNF-induced cytotoxicity directly depends on the AFP/TNF concentration ratio, demonstrating biphasic AFP activity. Our data show that 0.2 mg/ml of AFP acts synergistically to enhance cytotoxicity of suboptimal TNF doses. In contrast, the same AFP dose significantly attenuates the cytotoxicity of high TNF doses. It is concluded that AFP can function as a protective factor against TNF cytotoxicity in human hepatoma cells. These observations suggest that AFP secretion by certain tumor cells allows a highly flexible regulation of TNF cytotoxicity, dependent on the amount of endogenous AFP

 

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