Recombinant Human Growth/Differentiation Factor 5 Stimulates Mesenchyme Aggregation and Chondrogenesis Responsible for the Skeletal Development of Limbs
作者:
HöttenGertrud C.,
MatsumotoTomoaki,
KimuraMichio,
BechtoldRolf F.,
KronRenate,
OharaTakane,
TanakaHideyuki,
SatohYusuke,
OkazakiMakoto,
ShiraiTakuhiro,
PanHaiou,
KawaiShinji,
PohlJens S.,
KudoAkira,
期刊:
Growth Factors
(Taylor Available online 1996)
卷期:
Volume 13,
issue 1-2
页码: 65-74
ISSN:0897-7194
年代: 1996
DOI:10.3109/08977199609034567
出版商: Taylor&Francis
关键词: growth/differentiation factor;transforming growth factor-βsuperfamily;chondrogenesis;bone formation
数据来源: Taylor
摘要:
AbstractWe have expressed and biologically characterized recombinant human growth/differentiation factor 5 (huGDF5). This protein is composed of a mature homodimer consisting of 15 kD subunits. Using recombinant expressed protein, we have demonstrated that huGDFSin vitrostimulated mesenchyme aggregation and chondrogenesis in rat limb bud cells.In vivo, partially purified huGDF5 induced cartilage and bone formation in muscular tissues of rodents. However, in contrast to the effects of other BMPs, as for example BMP-2, the osteoblastic MC3T3-E1 cells did not respond to huGDF5 as measured by alkaline phosphatase activity. These results suggest that the action of GDF5 may be relatively specific for chondrogenesis during the entire process of the endochondral bone formation. GDF5 may control the morphogenesis of cartilaginous tissue, including joints, in the skeletal development of limbs.
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