Fractional Incorporation of Radionucleotides, a Marker of in vitro Tumor Cell Chemosensitivity in Colorectal Cancer
作者:
Fabrizio Franchi,
Patrizia Seminara,
Diana Giannarelli,
Nina Konovalova,
期刊:
Oncology
(Karger Available online 1991)
卷期:
Volume 48,
issue 6
页码: 510-516
ISSN:0030-2414
年代: 1991
DOI:10.1159/000226991
出版商: S. Karger AG
关键词: Tumor chemotherapy;Chemosensitivity assays;5-Fluorouracil;Mitomycin C;Colorectal cancer;Cell kinetics
数据来源: Karger
摘要:
Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four different drugs (5-FU, mitomycin C, 4’-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of 3H-TdR and 3H-UdR incorporation into DNA and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherap
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