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PCR‐SSO TYPING FOR DR4‐Dw SUBTYPES: APPLICATION TO UNRELATED BONE MARROW TRANSPLANT DONOR SELECTION

 

作者: T. M. Clay,   M. R. Howard,   J. L. Bidwell,   E. A. Bidwell,   P. A. Raymond,   J. E. Evans,   B. A. Bradley,  

 

期刊: International Journal of Immunogenetics  （WILEY Available online 1991）
卷期: Volume 18, issue 1‐2  

页码: 97-104

 

ISSN:1744-3121

 

年代: 1991

 

DOI:10.1111/j.1744-313X.1991.tb00009.x

 

出版商: Blackwell Publishing Ltd

 

数据来源: WILEY

 

摘要:

SUMMARYThirty‐seven DR4‐positive patient‐unrelated bone marrow donor pairs previously DR/DQ restriction fragment length polymorphism (RFLP) typed and tested in mixed lymphocyte culture (MLC), have been DR4‐Dw subtyped retrospectively using sequence specific oligonucleotide probes. We found that DR4‐Dw subtyping substantially increased the accuracy of pre‐MLC matching and could potentially accelerate donor searches by avoiding unnecessary MLC tests on Dw‐mism

 

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